Publication: Identification of a lysine-rich region of Fas as a raft nanodomain targeting signal necessary for Fas-mediated cell death.

Published in: Exp Cell Res 2010 May; 316(9): 1513-22

Authors: Rossin A, Kral R, Lounnas N, Chakrabandhu K, Mailfert S, Marguet D, Hueber AO

Summary

Fas interaction at the plasma membrane with its lipid and protein environment plays a crucial role in the early steps of Fas signalling induced by Fas ligand binding. Particularly, Fas localisation in the raft nanodomains, ezrin-mediated interaction with the actin cytoskeleton and subsequent internalization are critical steps in Fas-mediated cell death. We identified a lysine-rich region (LRR) in the cytoplasmic, membrane-proximal region of Fas as a key determinant modulating these initial events. Through a genetic approach, we demonstrate that Fas LRR represents another signal additional to palmitoylation targeting Fas to the raft nanodomains, and modulates Fas interaction with the cytoskeleton.

Link to Pubmed [PMID] – 20298688

Link to DOI – 10.1016/j.yexcr.2010.03.002