Publication: A versatile dual spot laser scanning confocal microscopy system for advanced fluorescence correlation spectroscopy analysis in living cell.

Published in: Rev Sci Instrum 2009 Aug; 80(8): 083702

Authors: Ferrand P, Pianta M, Kress A, Aillaud A, Rigneault H, Marguet D

Summary

A fluorescence correlation spectroscopy system based on two independent measurement volumes is presented. The optical setup and data acquisition hardware are detailed, as well as a complete protocol to control the location, size, and shape of the measurement volumes. A method that allows to monitor independently the excitation and collection efficiency distribution is proposed. Finally, a few examples of measurements that exploit the two spots in static and/or scanning schemes are reported.

Link to Pubmed [PMID] – 19725657

Link to DOI – 10.1063/1.3205447