Didier MARGUET – CIML

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Dorian BOURGEON

About

Member of the American Biophysical Society

Received his PhD in 1986 at the University of Grenoble (France). In 1988, he joined the CIML as
CNRS research scientist. From 1996 to 1998, he was appointed as visiting assistant professor at the John Hopkins
University. Since 2003, he heads a new team at the CIML, devoted to understanding the spatiotemporal organization of
molecular complexes in live cells based on biophotonic approaches.

Didier MARGUET has been contributing in the recent years to the development of new biophysical approaches and
tools to study cell membrane organization and dynamics. These studies revealed an organizational hierarchy at
different spatial scales for lipid nanodomains in intact cell membranes, and led to a new model in which
nanodomains work as “selective glue” rather than a “special organelle” by promoting temporary molecular
confinement.

Projects

LINkS -Long-range electrodynamic INteractions between proteins

Project: LINkS The LINkS project (EU FET-OPEN) aims to change the paradigms of self-organization of intracellular living matter, […]

D2OX – Distribution and Dynamics of OXPHOS

Project: D2OX The interdisciplinary D2OX project is divided into three highly interconnected tasks to elucidate how the efficiency of OXPHOS complexes […]

Publications

Perez-Martin E, Beranger T, Bonnet L, Teppe F, Lisauskas A, Ikamas K, Vrouwe E, Floriani E, Katona G..., Unveiling long-range forces in light-harvesting proteins: Pivotal roles of temperature and light. Sci Adv 2025 May; 11(18): eadv0346.

Mailfert S, Djendli M, Fabre R, Marguet D, Bertaux N, Quality control maps: Real-time quantitative control of single-molecule localization microscopy data. Biophys J 2025 Apr; 124(7): 1132-1145.

Jaeger M, Anastasio A, Chamy L, Brustlein S, Vincentelli R, Durbesson F, Gigan J, Thépaut M, Char R..., Light-inducible T cell engages trigger, tune, and shape the activation of primary T cells. Proc Natl Acad Sci USA 2023 Sep; 120(39): e2302500120.

Lechelon M, Meriguet Y, Gori M, Ruffenach S, Nardecchia I, Floriani E, Coquillat D, Teppe F, Mailfer..., Experimental evidence for long-distance electrodynamic intermolecular forces. Sci Adv 2022 Feb; 8(7): eabl5855.

Mailfert S, Wojtowicz K, Brustlein S, Blaszczak E, Bertaux N, Łukaszewicz M, Marguet D, Trombik T, Spot Variation Fluorescence Correlation Spectroscopy for Analysis of Molecular Diffusion at the Plasma Membrane of Living Cells. J Vis Exp 2020 Nov; (165): .

Chouaki Benmansour N, Ruminski K, Sartre AM, Phelipot MC, Salles A, Bergot E, Wu A, Chicanne G, Fall..., Author Correction: Phosphoinositides regulate the TCR/CD3 complex membrane dynamics and activation. Sci Rep 2020 Jul; 10(1): 12558.

Sarri B, Canonge R, Audier X, Simon E, Wojak J, Caillol F, Cador C, Marguet D, Poizat F, Giovannini ..., Fast stimulated Raman and second harmonic generation imaging for intraoperative gastrointestinal cancer detection. Sci Rep 2019 Jul; 9(1): 10052.

Mailfert S, Touvier J, Benyoussef L, Fabre R, Rabaoui A, Blache MC, Hamon Y, Brustlein S, Monneret S..., A Theoretical High-Density Nanoscopy Study Leads to the Design of UNLOC, a Parameter-free Algorithm., Biophys J 2018 Aug; 115(3): 565-576.

Scotté C, de Aguiar HB, Marguet D, Green EM, Bouzy P, Vergnole S, Winlove CP, Stone N, Rigneault H, Assessment of Compressive Raman versus Hyperspectral Raman for Microcalcification Chemical Imaging., Anal Chem 2018 Jun; 90(12): 7197-7203.

Wang R, Brustlein S, Mailfert S, Fabre R, Fallet M, Sivankutty S, Rigneault H, Marguet D, A straightforward STED-background corrected fitting model for unbiased STED-FCS analyses. Methods 2018 May; 140-141(): 212-222.

Nassima Chouaki-Benmansour, Kilian Ruminski, Anne-Marie Sartre, Marie-Claire Phelipot, Audrey Salles..., Phosphoinositides regulate the TCR/CD3 complex membrane dynamics and activation, <i>Scientific Reports</i>, 2018, 8 (1), pp.4966. <a target="_blank" href="https://dx.doi.org/10.1038/s41598-018-23109-8">⟨10.1038/s41598-018-23109-8⟩</a>.

Nardecchia I, Lechelon M, Gori M, Donato I, Preto J, Floriani E, Jaeger S, Mailfert S, Marguet D, Fe..., Detection of long-range electrostatic interactions between charged molecules by means of fluorescence correlation spectroscopy. Phys Rev E 2017 Aug; 96(2-1): 022403.

Mailfert S, Hamon Y, Bertaux N, He HT, Marguet D, A user's guide for characterizing plasma membrane subdomains in living cells by spot variation fluorescence correlation spectroscopy. Methods Cell Biol 2017; 139(): 1-22.

Blouin CM, Hamon Y, Gonnord P, Boularan C, Kagan J, Viaris de Lesegno C, Ruez R, Mailfert S, Bertaux..., Glycosylation-Dependent IFN-γR Partitioning in Lipid and Actin Nanodomains Is Critical for JAK Activation. Cell 2016 Aug; 166(4): 920-934.

Baffou G, Rigneault H, Marguet D, Jullien L, Reply to: “Validating subcellular thermal changes revealed by fluorescent thermosensors” and “The 10(5) gap issue between calculation and measurement in single-cell thermometry”. Nat Methods 2015 Sep; 12(9): 803.

Baffou G, Rigneault H, Marguet D, Jullien L, A critique of methods for temperature imaging in single cells. Nat Methods 2014 Sep; 11(9): 899-901.

Rouger V, Bordet G, Couillault C, Monneret S, Mailfert S, Ewbank JJ, Pujol N, Marguet D, Independent synchronized control and visualization of interactions between living cells and organisms. Biophys J 2014 May; 106(10): 2096-104.

Marquèze-Pouey B, Mailfert S, Rouger V, Goaillard JM, Marguet D, Physiological epidermal growth factor concentrations activate high affinity receptors to elicit calcium oscillations. PLoS One 2014; 9(9): e106803.

Rainy N, Chetrit D, Rouger V, Vernitsky H, Rechavi O, Marguet D, Goldstein I, Ehrlich M, Kloog Y, H-Ras transfers from B to T cells via tunneling nanotubes. Cell Death Dis 2013 Jul; 4(7): e726.

Billaudeau C, Mailfert S, Trombik T, Bertaux N, Rouger V, Hamon Y, He HT, Marguet D, Probing the plasma membrane organization in living cells by spot variation fluorescence correlation spectroscopy. Methods Enzymol 2013; 519(): 277-302.

Salles A, Billaudeau C, Sergé A, Bernard AM, Phélipot MC, Bertaux N, Fallet M, Grenot P, Marguet D..., Barcoding T cell calcium response diversity with methods for automated and accurate analysis of cell signals (MAAACS). PLoS Comput Biol 2013; 9(9): e1003245.

Rouger V, Bertaux N, Trombik T, Mailfert S, Billaudeau C, Marguet D, Sergé A, Mapping molecular diffusion in the plasma membrane by Multiple-Target Tracing (MTT). J Vis Exp 2012 May; (63): e3599.

Kress A, Ferrand P, Rigneault H, Trombik T, He HT, Marguet D, Brasselet S, Probing orientational behavior of MHC class I proteins and lipid probes in cell membranes by fluorescence polarization-resolved imaging. Biophys J 2011 Jul; 101(2): 468-76.

Malissen B, Marguet D, Last but not least., Nat Immunol 2011 Jun; 12(7): 592-3.

Brustlein S, Berto P, Hostein R, Ferrand P, Billaudeau C, Marguet D, Muir A, Knight J, Rigneault H, Double-clad hollow core photonic crystal fiber for coherent Raman endoscope., Opt Express 2011 Jun; 19(13): 12562-8.

Ruprecht V, Wieser S, Marguet D, Schütz GJ, Spot variation fluorescence correlation spectroscopy allows for superresolution chronoscopy of confinement times in membranes. Biophys J 2011 Jun; 100(11): 2839-45.

Brustlein S, Ferrand P, Walther N, Brasselet S, Billaudeau C, Marguet D, Rigneault H, Optical parametric oscillator-based light source for coherent Raman scattering microscopy: practical overview., J Biomed Opt 2011 Feb; 16(2): 021106.

He HT, Marguet D, Detecting nanodomains in living cell membrane by fluorescence correlation spectroscopy. Annu Rev Phys Chem 2011; 62(): 417-36.

Sophie Guia, Baptiste N Jaeger, Stefan Piatek, Sébastien Mailfert, Tomasz Trombik, Aurore Fenis, Ni..., Confinement of activating receptors at the plasma membrane controls natural killer cell tolerance. <i>Science Signaling</i>, 2011, 4 (167), pp.ra21. <a target="_blank" href="https://dx.doi.org/10.1126/scisignal.2001608">⟨10.1126/scisignal.2001608⟩</a>.

Guardiola-Serrano F, Rossin A, Cahuzac N, Lückerath K, Melzer I, Mailfert S, Marguet D, Zörnig M, ..., Palmitoylation of human FasL modulates its cell death-inducing function. Cell Death Dis 2010 Oct; 1(10): e88.

Rossin A, Kral R, Lounnas N, Chakrabandhu K, Mailfert S, Marguet D, Hueber AO, Identification of a lysine-rich region of Fas as a raft nanodomain targeting signal necessary for Fas-mediated cell death. Exp Cell Res 2010 May; 316(9): 1513-22.

Pierre Bon, Julien Savatier, Didier Marguet, Benoit Wattelier, Serge Monneret, Quantitative phase contrast, refractive index anisotropy measurement, GdR Ondes Day , 2010, Marseille, France.

Serge Monneret, Pierre Bon, Benoit Wattelier, Didier Marguet, Quantitative phase microscopy using a high resolution wavefront sensor, Second Japanese-French workshop together with the first American-French meeting on Nanobiophotonics , Oct 2009, Marseille, France.

Ferrand P, Pianta M, Kress A, Aillaud A, Rigneault H, Marguet D, A versatile dual spot laser scanning confocal microscopy system for advanced fluorescence correlation spectroscopy analysis in living cells. Rev Sci Instrum 2009 Aug; 80(8): 083702.

Chakrabandhu K, Huault S, Garmy N, Fantini J, Stebe E, Mailfert S, Marguet D, Hueber AO, The extracellular glycosphingolipid-binding motif of Fas defines its internalization route, mode and outcome of signals upon activation by ligand. Cell Death Differ 2008 Dec; 15(12): 1824-37.

Lenne PF, Rigneault H, Marguet D, Wenger J, Fluorescence fluctuations analysis in nanoapertures: physical concepts and biological applications. Histochem Cell Biol 2008 Nov; 130(5): 795-805.

Lasserre R, Guo XJ, Conchonaud F, Hamon Y, Hawchar O, Bernard AM, Soudja SM, Lenne PF, Rigneault H, ..., Raft nanodomains contribute to Akt/PKB plasma membrane recruitment and activation. Nat Chem Biol 2008 Sep; 4(9): 538-47.

Sergé A, Bertaux N, Rigneault H, Marguet D, Dynamic multiple-target tracing to probe spatiotemporal cartography of cell membranes. Nat Methods 2008 Aug; 5(8): 687-94.

He HT, Marguet D, T-cell antigen receptor triggering and lipid rafts: a matter of space and time scales. Talking Point on the involvement of lipid rafts in T-cell activation. EMBO Rep 2008 Jun; 9(6): 525-30.

Randriamampita C, Mouchacca P, Malissen B, Marguet D, Trautmann A, Lellouch AC, A novel ZAP-70 dependent FRET based biosensor reveals kinase activity at both the immunological synapse and the antisynapse. PLoS One 2008 Jan; 3(1): e1521.

Conchonaud F, Nicolas S, Amoureux MC, Ménager C, Marguet D, Lenne PF, Rougon G, Matarazzo V, Polysialylation increases lateral diffusion of neural cell adhesion molecule in the cell membrane. J Biol Chem 2007 Sep; 282(36): 26266-74.

Vera Preller, Annegret Gerber, Sabine Wrenger, Mauro Togni, Didier Marguet, Janine Tadje, Uwe Lendec..., TGF-beta1-mediated control of central nervous system inflammation and autoimmunity through the inhibitory receptor CD26. Journal of Immunology , 2007, 178 (7), pp.4632-40.

Preller V, Gerber A, Wrenger S, Togni M, Marguet D, Tadje J, Lendeckel U, Röcken C, Faust J, Neuber..., TGF-beta1-mediated control of central nervous system inflammation and autoimmunity through the inhibitory receptor CD26. J Immunol 2007 Apr; 178(7): 4632-40.

Wenger J, Conchonaud F, Dintinger J, Wawrezinieck L, Ebbesen TW, Rigneault H, Marguet D, Lenne PF, Diffusion analysis within single nanometric apertures reveals the ultrafine cell membrane organization. Biophys J 2007 Feb; 92(3): 913-9.

Chakrabandhu K, Hérincs Z, Huault S, Dost B, Peng L, Conchonaud F, Marguet D, He HT, Hueber AO, Palmitoylation is required for efficient Fas cell death signaling. EMBO J 2007 Jan; 26(1): 209-20.

Wenger J, Gérard D, Lenne PF, Rigneault H, Dintinger J, Ebbesen TW, Boned A, Conchonaud F, Marguet ..., Dual-color fluorescence cross-correlation spectroscopy in a single nanoaperture: towards rapid multicomponent screening at high concentrations. Opt Express 2006 Dec; 14(25): 12206-16.

Didier Marguet, [Photonic revolution or how nonlinear optics fosters new approaches in cell and tissue imaging], Medicine/Sciences , 2006, 22 (10), pp.787.

Djaker N, Marguet D, Rigneault H, [Stimulated Raman microscopy (CARS): From principles to applications]. Med Sci (Paris) 2006 Oct; 22(10): 853-8.

Marguet D, Galli T, [Photonic revolution or how nonlinear optics fosters new approaches in cell and tissue imaging]. Med Sci (Paris) 2006 Oct; 22(10): 787.

El Yacoubi M, Vaugeois JM, Marguet D, Sauze N, Guieu R, Costentin J, Fenouillet E, Behavioral characterization of CD26 deficient mice in animal tests of anxiety and antidepressant-like activity. Behav Brain Res 2006 Aug; 171(2): 279-85.

Marguet D, Lenne PF, Rigneault H, He HT, Dynamics in the plasma membrane: how to combine fluidity and order. EMBO J 2006 Aug; 25(15): 3446-57.

Lenne PF, Wawrezinieck L, Conchonaud F, Wurtz O, Boned A, Guo XJ, Rigneault H, He HT, Marguet D, Dynamic molecular confinement in the plasma membrane by microdomains and the cytoskeleton meshwork. EMBO J 2006 Jul; 25(14): 3245-56.

Popovici C, Fallet M, Marguet D, Birnbaum D, Roubin R, Intracellular trafficking of LET-756, a fibroblast growth factor of C. elegans, is controlled by a balance of export and nuclear signals. Exp Cell Res 2006 May; 312(9): 1484-95.

Cahuzac N, Baum W, Kirkin V, Conchonaud F, Wawrezinieck L, Marguet D, Janssen O, Zörnig M, Hueber A..., Fas ligand is localized to membrane rafts, where it displays increased cell death-inducing activity. Blood 2006 Mar; 107(6): 2384-91.

Guieu R, Fenouillet E, Devaux C, Fajloun Z, Carrega L, Sabatier JM, Sauze N, Marguet D, CD26 modulates nociception in mice via its dipeptidyl-peptidase IV activity. Behav Brain Res 2006 Jan; 166(2): 230-5.

Wenger J, Rigneault H, Dintinger J, Marguet D, Lenne PF, Single-fluorophore diffusion in a lipid membrane over a subwavelength aperture. J Biol Phys 2006 Jan; 32(1): SN1-4.

Preller V, Gerber A, Togni M, Wrenger S, Schraven B, Röcken C, Marguet D, Ansorge S, Brocke S, Rein..., CD26/DP IV in T cell activation and autoimmunity. Adv Exp Med Biol 2006; 575(): 187-93.

Wawrezinieck L, Rigneault H, Marguet D, Lenne PF, Fluorescence correlation spectroscopy diffusion laws to probe the submicron cell membrane organization. Biophys J 2005 Dec; 89(6): 4029-42.

He HT, Lellouch A, Marguet D, Lipid rafts and the initiation of T cell receptor signaling. Semin Immunol 2005 Feb; 17(1): 23-33.

Lelouard H, Ferrand V, Marguet D, Bania J, Camosseto V, David A, Gatti E, Pierre P, Dendritic cell aggresome-like induced structures are dedicated areas for ubiquitination and storage of newly synthesized defective proteins. J Cell Biol 2004 Mar; 164(5): 667-75.

Yan S, Marguet D, Dobers J, Reutter W, Fan H, Deficiency of CD26 results in a change of cytokine and immunoglobulin secretion after stimulation by pokeweed mitogen. Eur J Immunol 2003 Jun; 33(6): 1519-27.

Fan H, Yan S, Stehling S, Marguet D, Schuppaw D, Reutter W, Dipeptidyl peptidase IV/CD26 in T cell activation, cytokine secretion and immunoglobulin production. Adv Exp Med Biol 2003; 524(): 165-74.

Marguet D, Chimini G, The ABCA1 transporter and ApoA-I: obligate or facultative partners? Trends Cardiovasc Med 2002 Oct; 12(7): 294-8.

Wang M, Gorrell MD, Abbott CA, Jaggi R, Marguet D, Dickinson RG, Hepatic covalent adduct formation with zomepirac in the CD26-deficient mouse. J Gastroenterol Hepatol 2002 Jan; 17(1): 66-71.

Chambenoit O, Hamon Y, Marguet D, Rigneault H, Rosseneu M, Chimini G, Specific docking of apolipoprotein AI at the cell surface requires a functional ABCA1 transporter. J Biol Chem 2001 Mar; 276(13): 9955-60.

Hamon Y, Broccardo C, Chambenoit O, Luciani MF, Toti F, Chaslin S, Freyssinet JM, Devaux PF, McNeish..., ABC1 promotes engulfment of apoptotic cells and transbilayer redistribution of phosphatidylserine. Nat Cell Biol 2000 Jul; 2(7): 399-406.

Marguet D, Baggio L, Kobayashi T, Bernard AM, Pierres M, Nielsen PF, Ribel U, Watanabe T, Drucker DJ..., Enhanced insulin secretion and improved glucose tolerance in mice lacking CD26. Proc Natl Acad Sci USA 2000 Jun; 97(12): 6874-9.

Marguet D, Luciani MF, Moynault A, Williamson P, Chimini G, Engulfment of apoptotic cells involves the redistribution of membrane phosphatidylserine on phagocyte and prey. Nat Cell Biol 1999 Nov; 1(7): 454-6.

Marguet D, Spiliotis ET, Pentcheva T, Lebowitz M, Schneck J, Edidin M, Lateral diffusion of GFP-tagged H2Ld molecules and of GFP-TAP1 reports on the assembly and retention of these molecules in the endoplasmic reticulum. Immunity 1999 Aug; 11(2): 231-40.

Lebowitz MS, O'Herrin SM, Hamad AR, Fahmy T, Marguet D, Barnes NC, Pardoll D, Bieler JG, Schneck JP, Soluble, high-affinity dimers of T-cell receptors and class II major histocompatibility complexes: biochemical probes for analysis and modulation of immune responses. Cell Immunol 1999 Mar; 192(2): 175-84.

Hueber AO, Bernard AM, Battari CL, Marguet D, Massol P, Foa C, Brun N, Garcia S, Stewart C, Pierres ..., Thymocytes in Thy-1-/- mice show increased TCR signaling and impaired differentiation. Curr Biol 1997 Sep; 7(9): 705-8.

Guérin S, Mari B, Maulon L, Belhacène N, Marguet D, Auberger P, CD10 plays a specific role in early thymic development. FASEB J 1997 Apr; 11(5): 376-81.

Olcese L, Lang P, Vély F, Cambiaggi A, Marguet D, Bléry M, Hippen KL, Biassoni R, Moretta A, Moret..., Human and mouse killer-cell inhibitory receptors recruit PTP1C and PTP1D protein tyrosine phosphatases. J Immunol 1996 Jun; 156(12): 4531-4.

David F, Baricault L, Sapin C, Gallet X, Marguet D, Thomas-Soumarmon A, Trugnan G, Reduced cell surface expression of a mutated dipeptidyl peptidase IV (DPP IV/CD26) correlates with the generation of a beta strand in its C-terminal domain. Biochem Biophys Res Commun 1996 May; 222(3): 833-8.

Golstein P, Marguet D, Depraetere V, Fas bridging cell death and cytotoxicity: the reaper connection. Immunol Rev 1995 Aug; 146(): 45-56.

Golstein P, Marguet D, Depraetere V, Homology between reaper and the cell death domains of Fas and TNFR1. Cell 1995 Apr; 81(2): 185-6.

Bernard AM, Mattei MG, Pierres M, Marguet D, Structure of the mouse dipeptidyl peptidase IV (CD26) gene., Biochemistry 1994 Dec; 33(50): 15204-14.

Lazaro I, Naniche D, Signoret N, Bernard AM, Marguet D, Klatzmann D, Dragic T, Alizon M, Sattentau Q, Factors involved in entry of the human immunodeficiency virus type 1 into permissive cells: lack of evidence of a role for CD26. J Virol 1994 Oct; 68(10): 6535-46.

David F, Bernard AM, Pierres M, Marguet D, Identification of serine 624, aspartic acid 702, and histidine 734 as the catalytic triad residues of mouse dipeptidyl-peptidase IV (CD26). A member of a novel family of nonclassical serine hydrolases. J Biol Chem 1993 Aug; 268(23): 17247-52.

Malissen B, Wegener AM, Hoeveler A, Marguet D, Molecular dissection of the T-cell receptor/CD3 complex. Immunol Ser 1993; 59(): 29-40.

Fantino E, Marguet D, Lauquin GJ, Downstream activating sequence within the coding region of a yeast gene: specific binding in vitro of RAP1 protein. Mol Gen Genet 1992 Dec; 236(1): 65-75.

Darmoul D, Lacasa M, Baricault L, Marguet D, Sapin C, Trotot P, Barbat A, Trugnan G, Dipeptidyl peptidase IV (CD 26) gene expression in enterocyte-like colon cancer cell lines HT-29 and Caco-2. Cloning of the complete human coding sequence and changes of dipeptidyl peptidase IV mRNA levels during cell differentiation. J Biol Chem 1992 Mar; 267(7): 4824-33.

Marguet D, Bernard AM, Vivier I, Darmoul D, Naquet P, Pierres M, cDNA cloning for mouse thymocyte-activating molecule. A multifunctional ecto-dipeptidyl peptidase IV (CD26) included in a subgroup of serine proteases. J Biol Chem 1992 Feb; 267(4): 2200-8.

Vivier I, Marguet D, Naquet P, Bonicel J, Black D, Li CX, Bernard AM, Gorvel JP, Pierres M, Evidence that thymocyte-activating molecule is mouse CD26 (dipeptidyl peptidase IV). J Immunol 1991 Jul; 147(2): 447-54.

Gangloff SP, Marguet D, Lauquin GJ, Molecular cloning of the yeast mitochondrial aconitase gene (ACO1) and evidence of a synergistic regulation of expression by glucose plus glutamate. Mol Cell Biol 1990 Jul; 10(7): 3551-61.

Chimini G, Boretto J, Marguet D, Lanau F, Lauquin G, Pontarotti P, Molecular analysis of the human MHC class I region using yeast artificial chromosome clones. Immunogenetics 1990; 32(6): 419-26.

Marguet D, Guo XJ, Lauquin GJ, Yeast gene SRP1 (serine-rich protein). Intragenic repeat structure and identification of a family of SRP1-related DNA sequences. J Mol Biol 1988 Aug; 202(3): 455-70.

Marguet D, Fantino E, Lauquin GJ, Gene cloning from yeast chromosome-specific mini-library. Isolation of the SRP1-related DNA sequence located on chromosome XV. FEBS Lett 1988 Jul; 234(2): 421-5.

Marguet D, Lauquin GJ, The yeast SRP gene: positive modulation by glucose of its transcriptional expression. Biochem Biophys Res Commun 1986 Jul; 138(1): 297-303.