Immunobiologie des cellules stromales – CIML

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Theme

Innate immunity at mucosal sites

Members

Research Director

Johnny BONNARDEL

Research Officer

Mathieu COURIVAUD

Cyrille MIONNET

Research Officer

Alexia ISSARTEL

Betty BROUSSEAU

About

Stromal cells are connective tissue cells found in all our organs. In lymphoid organs such as lymph nodes, they provide a migratory and feeding platform for leukocytes. In non-lymphoid organs, they are best known for supporting the function of parenchymal cells. However, considerable evidence suggests that stromal cells also control the homeostasis of tissue-resident immune cells, such as macrophages.

Our laboratory studies the immunoregulatory functions of stromal cells in lymphoid organs and the dialogue between stromal cells and macrophages in non-lymphoid organs.

Projects

Circulating precursors and niche requirements for LysoDC differentiation (LysoDiff)

Our health depends on a delicate balance between immune defense against pathogens and tolerance to […]

Hugues LELOUARD

Accelerating our ability to understand and combat immunopathology caused by respiratory infections due to emerging viruses: from mouse models to patients to macaques

Project: UNFIRE Other participants: Ciphe (Ana Zarubica), Hospices Civils de Lyon and CIRI (Sophie Trouillet-Assant, Thierry Walzer), CoVir team […]

Philippe PIERRE

Intracellular Carriers Against Resistant microOrganisms (ICARO)

Project: ICARO Current acellular vaccines are mostly effective against extracellular pathogens, but fail to induce a response […]

Hugues LELOUARD

Cyrille MIONNET

Project: Myfunbat The project aims to define the diversity and functions of brown adipose tissue (BAT) macrophages, […]

Project: EndoPos The EndoPos project explores the role of the GDPD2 enzyme, produced in particular by pulp fibroblasts […]

Project: Path Finder This project aims to characterize the spatio-temporal development of type I dendritic cells (cDC1) in […]

Project: T-MAC The T-MAC project aims to understand how testicular macrophages and their niches contribute to the maintenance of […]

Project: NICHE The NICHE project explores the concept that macrophages (Macs) depend on local niches formed by […]

Project: HOMEOSTASIS HOMEOSTASIS explores how host–commensal interactions preserve tissue equilibrium. Using a model of long-term, inflammation-free oral colonization by […]

Johnny BONNARDEL

Project: CARCINOMAC Head and neck cancers are the sixth most common type of cancer. Among these, the Oral Squamous […]

Johnny BONNARDEL

The ocular surface is protected by a multi-layered tear film, with the lipid layer produced by Meibomian glands (MGs) playing […]

Johnny BONNARDEL

Publications

Tauber M, Basso L, Martin J, Bostan L, Pinto MM, Thierry GR, Houmadi R, Serhan N, Loste A, Blériot ..., Correction: Landscape of mast cell populations across organs in mice and humans. J Exp Med 2024 Feb; 221(2): .

Sekias M, Baratin M, Bajenoff M, Isolation and Characterization of Testis Macrophages Using Flow Cytometry. Methods Mol Biol 2024; 2713(): 199-206.

McKendrick JG, Jones GR, Elder SS, Watson E, T'Jonck W, Mercer E, Magalhaes MS, Rocchi C, Hegarty LM..., CSF1R-dependent macrophages in the salivary gland are essential for epithelial regeneration after radiation-induced injury. Sci Immunol 2023 Nov; 8(89): eadd4374.

Garcia-Seyda N, Song S, Seveau de Noray V, David-Broglio L, Matti C, Artinger M, Dupuy F, Biarnes-Pe..., Naive T lymphocytes chemotax long distance to CCL21 but not to a source of bioactive S1P., iScience 2023 Oct; 26(10): 107695.

Tauber M, Basso L, Martin J, Bostan L, Pinto MM, Thierry GR, Houmadi R, Serhan N, Loste A, Blériot ..., Landscape of mast cell populations across organs in mice and humans. J Exp Med 2023 Oct; 220(10): .

Ugur M, Labios RJ, Fenton C, Knöpper K, Jobin K, Imdahl F, Golda G, Hoh K, Grafen A, Kaisho T, Sali..., Lymph node medulla regulates the spatiotemporal unfolding of resident dendritic cell networks. Immunity 2023 Aug; 56(8): 1778-1793.e10.

Kaltenbach L, Martzloff P, Bambach SK, Aizarani N, Mihlan M, Gavrilov A, Glaser KM, Stecher M, Thün..., Slow integrin-dependent migration organizes networks of tissue-resident mast cells. Nat Immunol 2023 Jun; 24(6): 915-924.

Chia SL, Kapoor S, Carvalho C, Bajénoff M, Gentek R, Mast cell ontogeny: From fetal development to life-long health and disease. Immunol Rev 2023 May; 315(1): 31-53.

Baudon EM, Bajenoff M, Tingible body macrophages: Gargantuan chameleons of the germinal center. J Exp Med 2023 Apr; 220(4): .

Baudon EM, Bajenoff M, [Tingible body macrophages: Hungry predators serving immunity]. Med Sci (Paris) 2023; 39(8-9): 615-617.

Menzel L, Zschummel M, Crowley T, Franke V, Grau M, Ulbricht C, Hauser A, Siffrin V, Bajénoff M, Ac..., Lymphocyte access to lymphoma is impaired by high endothelial venue regression. Cell Rep 2021 Oct; 37(4): 109878.

Blériot C, Barreby E, Dunsmore G, Ballaire R, Chakarov S, Ficht X, De Simone G, Andreata F, Fumagal..., A subset of Kupffer cells regulates metabolism through the expression of CD36. Immunity 2021 Sep; 54(9): 2101-2116.e6.

De Simone G, Andreata F, Bleriot C, Fumagalli V, Laura C, Garcia-Manteiga JM, Di Lucia P, Gilotto S,..., Identification of a Kupffer cell subset capable of reverting the T cell dysfunction induced by hepatocellular priming. Immunity 2021 Sep; 54(9): 2089-2100.e8.

Bellomo A, Gentek R, Golub R, Bajénoff M, Macrophage-fibroblast circuits in the spleen. Immunol Rev 2021 Jul; 302(1): 104-125.

Bijnen M, Bajénoff M, Gland Macrophages: Reciprocal Control and Function within Their Niche. Trends Immunol 2021 Feb; 42(2): 120-136.

Garcia-Seyda N, Seveau V, Manca F, Biarnes-Pelicot M, Valignat MP, Bajénoff M, Theodoly O, Human neutrophils swim and phagocytose bacteria. Biol Cell 2021 Jan; 113(1): 28-38.

Lacerda Mariano L, Rousseau M, Varet H, Legendre R, Gentek R, Saenz Coronilla J, Bajenoff M, Gomez P..., Functionally distinct resident macrophage subsets differentially shaped responses to infection in the bladder. Sci Adv 2020 Nov; 6(48): .

Zeis P, Lian M, Fan X, Herman JS, Hernandez DC, Gentek R, Elias S, Symowski C, Knöpper K, Peltokang..., In Situ Maturation and Tissue Adaptation of Type 2 Innate Lymphoid Cell Progenitors. Immunity 2020 Oct; 53(4): 775-792.e9.

Krivanek J, Soldatov RA, Kastriti ME, Chontorotzea T, Herdina AN, Petersen J, Szarowska B, Landova M..., Dental cell type atlas reveals stem and differentiated cell types in mouse and human teeth. Nat Commun 2020 Sep; 11(1): 4816.

Simic M, Manosalva I, Spinelli L, Gentek R, Shayan RR, Siret C, Girard-Madoux M, Wang S, de Fabritus..., Distinct Waves from the Hemogenic Endothelium Give Rise to Layered Lymphoid Tissue Inducer Cell Ontogeny, Cell Rep 2020 Aug; 32(6): 108004.

Alicia Bellomo, Isabelle Mondor, Lionel Spinelli, Marine Lagueyrie, Benjamin J Stewart, Nicolas Brou..., Reticular Fibroblasts Expressing the Transcription Factor WT1 Define a Stromal Niche that Maintains and Replenishes Splenic Red Pulp Macrophages, <i>Immunity</i>, 2020, 53 (1), pp.127 - 142.e7. <a target="_blank" href="https://dx.doi.org/10.1016/j.immuni.2020.06.008">⟨10.1016/j.immuni.2020.06.008⟩</a>.

Garcia-Seyda N, Aoun L, Tishkova V, Seveau V, Biarnes-Pelicot M, Bajénoff M, Valignat MP, Theodoly ..., Microfluidic device to study flow-free chemotaxis of swimming cells. Lab Chip 2020 May; 20(9): 1639-1647.

Etzerodt A, Moulin M, Doktor TK, Delfini M, Mossadegh-Keller N, Bajenoff M, Sieweke MH, Moestrup SK,..., Tissue-resident macrophages in omentum promote metastatic spread of ovarian cancer. J Exp Med 2020 Apr; 217(4): .

Guilliams M, Thierry GR, Bonnardel J, Bajenoff M, Establishment and Maintenance of the Macrophage Niche. Immunity 2020 Mar; 52(3): 434-451.

Stewart BJ, Ferdinand JR, Young MD, Mitchell TJ, Loudon KW, Riding AM, Richoz N, Frazer GL, Stanifor..., Spatiotemporal immune zonation of the human kidney. Science 2019 Sep; 365(6460): 1461-1466.

BONNARDEL J, T'Jonck W, Gaublomme D, Browaeys R, Scott CL, Martens L, Vanneste B, De Prijck S, Nedo..., Stellate Cells, Hepatocytes, and Endothelial Cells Imprint the Kupffer Cell Identity on Monocytes Colonizing the Liver Macrophage Niche, Immunity.

Mondor I, Baratin M, Lagueyrie M, Saro L, Henri S, Gentek R, Suerinck D, Kastenmuller W, Jiang JX, B..., Lymphatic Endothelial Cells Are Essential Components of the Subcapsular Sinus Macrophage Niche. Immunity 2019 Jun; 50(6): 1453-1466.e4.

Thierry GR, Gentek R, Bajenoff M, Remodeling of reactive lymph nodes: Dynamics of stromal cells and underlying chemokine signaling. Immunol Rev 2019 May; 289(1): 42-61.

Balzano M, De Grandis M, Vu Manh TP, Chasson L, Bardin F, Farina A, Sergé A, Bidaut G, Charbord P, ..., Nidogen-1 Contributes to the Interaction Network Involved in Pro-B Cell Retention in the Peri-sinusoidal Hematopoietic Stem Cell Niche. Cell Rep 2019 Mar; 26(12): 3257-3271.e8.

Chakarov S, Lim HY, Tan L, Lim SY, See P, Lum J, Zhang XM, Foo S, Nakamizo S, Duan K, Kong WT, Gente..., Two distinct interstitial macrophage populations coexist across tissues in specific subtissular niches. Science 2019 Mar; 363(6432): .

Cabeza-Cabrerizo M, van Blijswijk J, Wienert S, Heim D, Jenkins RP, Chakravarty P, Rogers N, Frederi..., Tissue clonality of dendritic cell subsets and emergency DCpoiesis revealed by multicolor fate mapping of DC progenitors. Sci Immunol 2019 Mar; 4(33): .

Gentek R, Ghigo C, Hoeffel G, Jorquera A, Msallam R, Wienert S, Klauschen F, Ginhoux F, Bajénoff M, Correction: Epidermal γδ T cells originate from yolk sac hematopoiesis and clonally self-renew in the adult. J Exp Med 2018 Dec; 215(12): 3213.

Thierry GR, Kuka M, De Giovanni M, Mondor I, Brouilly N, Iannacone M, Bajénoff M, The conduit system exports locally secreted IgM from lymph nodes. J Exp Med 2018 Dec; 215(12): 2972-2983.

Gentek R, Ghigo C, Hoeffel G, Jorquera A, Msallam R, Wienert S, Klauschen F, Ginhoux F, Bajénoff M, Epidermal γδ T cells originate from yolk sac hematopoiesis and clonally self-renewed in the adult. J Exp Med 2018 Dec; 215(12): 2994-3005.

Pezoldt J, Pasztoi M, Zou M, Wiechers C, Beckstette M, Thierry GR, Vafadarnejad E, Floess S, Arampat..., Neonatally imprinted stromal cell subsets induce tolerogenic dendritic cells in mesenteric lymph nodes. Nat Commun 2018 Sep;9(1):3903.

Bellomo A, Gentek R, Bajénoff M, Baratin M, Lymph node macrophages: Scavengers, immune sentinels and trophic effectors. Cell Immunol 2018 Aug; 330(): 168-174.

Gentek R, Ghigo C, Hoeffel G, Bulle MJ, Msallam R, Gautier G, Launay P, Chen J, Ginhoux F, Bajénoff..., Hemogenic Endothelial Fate Mapping Reveals Dual Developmental Origin of Mast Cells. Immunity 2018 Jun; 48(6): 1160-1171.e5.

Baranska A, Shawket A, Jouve M, Baratin M, Malosse C, Voluzan O, Vu Manh TP, Fiore F, Bajénoff M, B..., Unveiling skin macrophage dynamics explains both tattoo persistence and strenuous removal. J Exp Med 2018 Apr; 215(4): 1115-1133.

Ghigo C, Gentek R, Bajénoff M, Imaging the Lymph Node Stroma. Methods Mol Biol 2018; 1763(): 53-61.

Habbeddine M, Verthuy C, Rastoin O, Chasson L, Bebien M, Bajenoff M, Adriouch S, den Haan JMM, Penni..., Receptor Activator of NF-κB Orchestrates Activation of Antiviral Memory CD8 T Cells in the Spleen Marginal Zone. Cell Rep 2017 Nov; 21(9): 2515-2527.

Abou-Hamdan M, Gharib B, Bajenoff M, Julia V, de Reggi M, Pantethine Down-Regulates Leukocyte Recruitment and Inflammatory Parameters in a Mouse Model of Allergic Airway Inflammation. Med Sci Monit Basic Res 2017 Nov; 23(): 368-372.

Baratin M, Simon L, Jorquera A, Ghigo C, Dembele D, Nowak J, Gentek R, Wienert S, Klauschen F, Malis..., T Cell Zone Resident Macrophages Silently Dispose of Apoptotic Cells in the Lymph Node. Immunity 2017 Aug; 47(2): 349-362.e5.

Gentek R, Bajénoff M, Lymph Node Stroma Dynamics and Approaches for Their Visualization. Trends Immunol 2017 Apr; 38(4): 236-247.

Mondor I, Jorquera A, Sene C, Adriouch S, Adams RH, Zhou B, Wienert S, Klauschen F, Bajénoff M, Clonal Proliferation and Stochastic Pruning Orchestrate Lymph Node Vasculature Remodeling. Immunity 2016 Oct; 45(4): 877-888.

Ardouin L, Luche H, Chelbi R, Carpentier S, Shawket A, Montanana Sanchis F, Santa Maria C, Grenot P,..., Broad and Largely Concordant Molecular Changes Characterize Tolerogenic and Immunogenic Dendritic Cell Maturation in Thymus and Periphery. Immunity 2016 Aug; 45(2): 305-18.

Terhorst D, Chelbi R, Wohn C, Malosse C, Tamoutounour S, Jorquera A, Bajenoff M, Dalod M, Malissen B..., Dynamics and Transcriptomics of Skin Dendritic Cells and Macrophages in an Imiquimod-Induced, Biphasic Mouse Model of Psoriasis. J Immunol 2015 Nov; 195(10): 4953-61.

Molawi K, Wolf Y, Kandalla PK, Favret J, Hagemeyer N, Frenzel K, Pinto AR, Klapproth K, Henri S, Mal..., Progressive replacement of embryo-derived cardiac macrophages with age. J Exp Med 2014 Oct; 211(11): 2151-8.

Jarjour M, Jorquera A, Mondor I, Wienert S, Narang P, Coles MC, Klauschen F, Bajénoff M, Fate mapping reveals origin and dynamics of lymph node follicular dendritic cells. J Exp Med 2014 Jun;211(6):1109-22.

Mionnet C, Mondor I, Jorquera A, Loosveld M, Maurizio J, Arcangeli ML, Ruddle NH, Nowak J, Aurrand-L..., Identification of a new stromal cell type involved in the regulation of inflamed B cell follicles. PLoS Biol 2013 Oct; 11(10): e1001672.

Ghigo C, Mondor I, Jorquera A, Nowak J, Wienert S, Zahner SP, Clausen BE, Luche H, Malissen B, Klaus..., Multicolor fate mapping of Langerhans cell homeostasis. J Exp Med 2013 Aug; 210(9): 1657-64.

Mancino A, Habbeddine M, Johnson E, Luron L, Bebien M, Memet S, Fong C, Bajenoff M, Wu X, Karin M, C..., I kappa B kinase alpha (IKKα) activity is required for functional maturation of dendritic cells and acquired immunity to infection. EMBO J 2013 Mar; 32(6): 816-28.

Baptiste N Jaeger, Jean Donadieu, Céline Cognet, Claire Bernat, Diana Ordoñez-Rueda, Vincent Barlo..., Neutrophil depletion odd natural killer cell maturation, function, and homeostasis. <i>Journal of Experimental Medicine</i>, 2012, 209 (3), pp.565-80. <a target="_blank" href="https://dx.doi.org/10.1084/jem.20111908">⟨10.1084/jem.20111908⟩</a>.

Bajénoff M, Stromal cells control soluble material and cellular transport in lymph nodes. Front Immunol 2012; 3(): 304.

Mionnet C, Sanos SL, Mondor I, Jorquera A, Laugier JP, Germain RN, Bajénoff M, High endothelialvenules as traffic control points maintaining lymphocyte population homeostasis in lymph nodes. Blood 2011 Dec; 118(23): 6115-22.

Frontera V, Arcangeli ML, Zimmerli C, Bardin F, Obrados E, Audebert S, Bajenoff M, Borg JP, Aurrand-..., Cutting edge: JAM-C controls homeostatic chemokine secretion in lymph node fibroblastic reticular cells expressing thrombomodulin. J Immunol 2011 Jul; 187(2): 603-7.

Sanos SL, Nowak J, Fallet M, Bajenoff M, Stromal cell networks regulate thymocyte migration and dendritic cell behavior in the thymus. J Immunol 2011 Mar; 186(5): 2835-41.

Bajénoff M, Germain RN, B-cell follicle development remodels the conduit system and allows soluble antigen delivery to follicular dendritic cells. Blood 2009 Dec; 114(24): 4989-97.

Klauschen F, Ishii M, Qi H, Bajénoff M, Egen JG, Germain RN, Meier-Schellersheim M, Quantifying cellular interaction dynamics in 3D fluorescence microscopy data. Nat Protoc 2009; 4(9): 1305-11.

Bajénoff M, Glaichenhaus N, Germain RN, Fibroblastic reticular cells guide T lymphocyte entry into and migration within the splenic T cell zone. J Immunol 2008 Sep; 181(6): 3947-54.

Germain RN, Bajénoff M, Castellino F, Chieppa M, Egen JG, Huang AY, Ishii M, Koo LY, Qi H, Making friends in out-of-the-way places: how cells of the immune system get together and how they conduct their business as revealed by intravital imaging. Immunol Rev 2008 Feb; 221(): 163-81.

Bajénoff M, Germain RN, Seeing is believing: a focus on the contribution of microscopic imaging to our understanding of immune system function. Eur J Immunol 2007 Nov; 37 Suppl 1(): S18-33.

Bajénoff M, Egen JG, Qi H, Huang AY, Castellino F, Germain RN, Highways, byways and breadcrumbs: directing lymphocyte traffic in the lymph node. Trends Immunol 2007 Aug; 28(8): 346-52.

Bajénoff M, [Lymphocyte migration in stromal cell networks]. Med Sci (Paris) 2007 Apr; 23(4): 345-7.

Bajénoff M, Egen JG, Koo LY, Laugier JP, Brau F, Glaichenhaus N, Germain RN, Stromal cell networks regulate lymphocyte entry, migration, and territoriality in lymph nodes. Immunity 2006 Dec; 25(6): 989-1001.

Bajénoff M, Breart B, Huang AY, Qi H, Cazareth J, Braud VM, Germain RN, Glaichenhaus N, Natural killer cell behavior in lymph nodes revealed by static and real-time imaging. J Exp Med 2006 Mar; 203(3): 619-31.

Wurtz O, Bajénoff M, Guerder S, IL-4-mediated inhibition of IFN-gamma production by CD4+ T cells proceeds by several developmentally regulated mechanisms. Int Immunol 2004 Mar; 16(3): 501-8.

Bajénoff M, Guerder S, Homing to nonlymphoid tissues is not necessary for effector Th1 cell differentiation. J Immunol 2003 Dec; 171(12): 6355-62.

Bajénoff M, Granjeaud S, Guerder S, The strategy of T cell antigen-presenting cell encounter in antigen-draining lymph nodes revealed by imaging of initial T cell activation. J Exp Med 2003 Sep; 198(5): 715-24.

Bajénoff M, Wurtz O, Guerder S, Repeated antigen exposure is necessary for the differentiation, but not the initial proliferation, of naive CD4(+) T cells. J Immunol 2002 Feb; 168(4): 1723-9.

Funding