Romain RONCAGALLI – CIML

Search

Theme

About

R. Roncagalli obtained his PhD in molecular biology in 2007 in André Veillette's laboratory at the University of Montreal (Canada). There, he studied new classes of molecular adaptors (SAP family) and receptors (SLAM family) and demonstrated the fundamental biological role of these proteins in NK cells.
As a postdoctoral researcher, he then joined the team of Bernard and Marie Malissen at the Marseille-Luminy Immunology Center (CIML) to work on T cell signaling. In 2010, R. Roncagalli obtained a permanent position as a research fellow (CR) at the CNRS to develop and study experimental models that have largely contributed to changing our fundamental understanding of T cell activation.
Since 2024, Romain Roncagalli has been head of the “Integrative Biology of T Lymphocytes” team, which works on the molecular mechanisms behind T lymphocyte activation using experimental approaches combining murine genetics, systems biology, modeling and mass spectrometry analyses.

Publications

Zhang F, Celis-Gutierrez J, Zhang L, Mellado V, Gelard L, Panigot S, Mori D, Lu L, Voisinne G, Vilar..., A CARMIL2 gain-of-function mutation suffices to trigger most CD28 costimulatory functions in vivo. J Exp Med 2025 Aug; 222(8): .

Ruminski K, Celis-Gutierrez J, Jarmuzynski N, Maturin E, Audebert S, Malissen M, Camoin L, Voisinne ..., Mapping the SLP76 interactome in T cells lacking each of the GRB2-family adapters reveals molecular plasticity of the TCR signaling pathway. Front Immunol 2023; 14(): 1139123.

Voisinne G, Locard-Paulet M, Froment C, Maturin E, Menoita MG, Girard L, Mellado V, Burlet-Schiltz O..., Kinetic proofreading through the multi-step activation of the ZAP70 kinase underlies early T cell ligand discrimination. Nat Immunol 2022 Sep; 23(9): 1355-1364.

Li B, Lu Y, Zhong MC, Qian J, Li R, Davidson D, Tang Z, Zhu K, Argenty J, de Peredo AG, Malissen B, ..., Cis interactions between CD2 and its ligands on T cells are required for T cell activation. Sci Immunol 2022 Aug; 7(74): eabn6373.

Zhai Y, Celis-Gutierrez J, Voisinne G, Mori D, Girard L, Burlet-Schiltz O, de Peredo AG, Roncagalli ..., Opposing regulatory functions of the TIM3 (HAVCR2) signalosome in primary effector T cells as revealed by quantitative interactomics. Cell Mol Immunol 2021 Jun; 18(6): 1581-1583.

Mori D, Grégoire C, Voisinne G, Celis-Gutierrez J, Aussel R, Girard L, Camus M, Marcellin M, Argent..., The T cell CD6 receptor operates a multitask signalosome with opposite functions in T cell activation. J Exp Med 2021 Feb; 218(2): .

Locard-Paulet M, Voisinne G, Froment C, Goncalves Menoita M, Ounoughene Y, Girard L, Gregoire C, Mor..., LymphoAtlas: a dynamic and integrated phosphoproteomic resource of TCR signaling in primary T cells reveals ITSN2 as a regulator of effector functions. Mol Syst Biol 2020 Jul; 16(7): e9524.

Voisinne G, Kersse K, Chaoui K, Lu L, Chaix J, Zhang L, Goncalves Menoita M, Girard L, Ounoughene Y,..., Quantitative interactomics in primary T cells reveals TCR signal diversification extent and dynamics. Nat Immunol 2019 Nov; 20(11): 1530-1541.

Celis-Gutierrez J, Blattmann P, Zhai Y, Jarmuzynski N, Ruminski K, Grégoire C, Ounoughene Y, Fiore ..., Quantitative Interactomics in Primary T Cells Provides a Rationale for Concomitant PD-1 and BTLA Coinhibitor Blockade in Cancer Immunotherapy. Cell Rep 2019 Jun; 27(11): 3315-3330.e7.

Stephanie Gon, Marie Loosveld, Thomas Crouzet, Delphine Potier, Melanie Bonnet, Stephanie Morin, ..., Fit αβ T-Cell Receptor suppresses leukemogenesis of Pten-deficient thymocytes. <i>Haematologica</i>, 2018, <a target="_blank" href="https://dx.doi.org/10.3324/haematol.2018.188359">⟨10.3324/haematol.2018.188359⟩</a>.

Roncagalli R, Cucchetti M, Jarmuzynski N, Grégoire C, Bergot E, Audebert S, Baudelet E, Menoita MG,..., The scaffolding function of the RLTPR protein explains its essential role for CD28 co-stimulation in mouse and human T cells. J Exp Med 2016 Oct; 213(11): 2437-2457.

Voisinne G, García-Blesa A, Chaoui K, Fiore F, Bergot E, Girard L, Malissen M, Burlet-Schiltz O, Go..., Co-recruitment analysis of the CBL and CBLB signalosomes in primary T cells identifies CD5 as a key regulator of TCR-induced ubiquitylation. Mol Syst Biol 2016 Jul; 12(7): 876.

Bounab Y, Anne-Marie-Hesse, Iannascoli B, Grieco L, Couté Y, Niarakis A, Roncagalli R, Lie E, Lam ..., Proteomic Analysis of the SH2 Domain-containing Leukocyte Protein of 76 kDa (SLP76) Interactome in Resting and Activated Primary Mast Cells. Mol Cell Proteomics 2014 Feb; 13(2): 678.

Liang Y, Cucchetti M, Roncagalli R, Yokosuka T, Malzac A, Bertosio E, Imbert J, Nijman IJ, Suchanek ..., The lymphoid lineage-specific actin-uncapping protein Rltpr is essential for costimulation via CD28 and the development of regulatory T cells. Nat Immunol 2013 Aug; 14(8): 858-66.

Roncagalli R, Mingueneau M, Grégoire C, Langlet C, Malissen B, Malissen M, Lymphoproliferative disorders involving T helper effector cells with defective LAT signalosomes. Semin Immunopathol 2010 Jun; 32(2): 117-25.

Mingueneau M, Sansoni A, Grégoire C, Roncagalli R, Aguado E, Weiss A, Malissen M, Malissen B, The proline-rich sequence of CD3epsilon controls T cell antigen receptor expression on and signaling potency in preselection CD4+CD8+ thymocytes. Nat Immunol 2008 May; 9(5): 522-32.