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About

Carrière (CRHC, INSERM)

After a master's degree in immunology, I completed my doctoral training in the laboratory of Dr. Anne-Marie SCHMITT-VERHULST, at the Marseille-Luminy Immunology Center (CIML), in Marseille, on a project aimed at understanding the functional association between the T cell receptor and the CD8 co-receptor during T lymphocyte activation and central tolerance induction.

Then I joined the University of California, San Diego, in the United States. In the laboratory of Professor Michael Karin, I deciphered the molecular basis of NF-kB inhibition by glucocorticoids. (Science 270, 286; PMID: 7569976; with a dedicated commentary, Science 270, 232; PMID: 7569969).

I was then recruited by Inserm as a researcher in the team of Dr. Anne-Marie SCHMITT-VERHULST, CIML, where I led projects exploring the molecular bases of the differentiation of naive CD8 T lymphocytes into cytolytic effector cells. I thus demonstrated that the expression of an active form of the STAT5 transcription factor in CD8 T lymphocytes potentiates (i) their capacity to infiltrate induced melanomas, and (ii) their anti-tumor functions (tumor-specific cytotoxicity, resistance to immunosuppression).

In 2015, I joined the team of Dr. Toby LAWRENCE, CIML, where I led a project demonstrating the central role of the IKKb/NF-kB signaling pathway in melanomagenesis and melanoma immune surveillance. I also co-supervised a project characterizing the role of the AP-1 factor Jun in CSF1-dependent differentiation of monocyte-derived macrophages and the specification of pro-tumor macrophages.

From October 2025, I will join the team of Dr. Marc DALOD, CIML, where I will continue the analysis of the modulation of melanoma immunogenicity by IKKb/NF-kB signaling. I will also participate in an analysis of the contribution of dendritic cell subpopulations to anti-tumor responses mediated by NK cells and CD8 T lymphocytes.

Expertise

• Immunologie ; Immunothérapies ; Immunophénotypage en cytométrie de flux, conventionnelle et spectrale ; Mélanomes et microenvironnement tumoral.
• 50 publications (N. Auphan jusqu’en 2000 ; N. Auphan-Anezin à partir de 2001)
4,593 citations in total; H index = 27
Identifiers: ORCID 0000-0002-1967-5206; Web of Science Researcher E-7790-2018
• Encadrement : 3 post-doctorants, 5 doctorants, 12 étudiants master, 8 ingénieurs.

Institutional external missions

Appointed member of National Commission 1, Onco-Immunology, of the ARC Foundation: 01/2019 – 12/2026

Missions transverses internes

• Coordinatrice scientifique de la plateforme de “Bio-imagerie non invasive”.
• Membre du comité de pilotage de la plateforme de “Cytométrie en flux”.
• Membre du SBEA: immunité anti-tumorale et modèles murins précliniques.
• Membre du conseil de laboratoire dont présidence (2022-2023).

Projects

Project
2025
Contributions of non-coding genome-derived antigens to anti-tumor immune responses.

Project: PLBIO-25 Coordinator: Marianne BURBAGE, Institut Curie, Paris. Other participant: Andrew GRIFFITHS, ESPCI, Paris. The effectiveness of CD8 T lymphocytes […]

Nathalie AUPHAN-ANEZIN, PhD, HDR
Project
2025
To determine how dendritic cells promote effective anti-tumor NK and CD8 T responses in mouse models of breast cancer, spontaneously or in response to adjuvant administration

Project: ACLAMADABREC Partly through their effectiveness in activating natural killer (NK) cells and CD8 T lymphocytes […]

Marc DALOD
Project
2025
International Research Project: Therapeutic role of macrophages in oncology and regeneration

Project: CureMAC This Franco-German research project, coordinated by Sandrine Sarrazin, combines the expertise of her group with that of the teams […]

Sandrine SARRAZIN

Publications

2021
Ghislat G, Cheema AS, Baudoin E, Verthuy C, Ballester PJ, Crozat K, Attaf N, Dong C, Milpied P, Mali..., NF-κB-dependent IRF1 activation programs cDC1 dendritic cells to drive antitumor immunity. Sci Immunol 2021 Jul; 6(61): .
2020
Jennifer Bintz, Analía Meilerman Abuelafia, François Gerbe, Elodie Baudoin, Nathalie Auphan-Anezin..., Expression of POU2F3 Transcription Factor Controls Inflammation, Immunological Recruitment and Metastasis of Pancreatic Cancer in Mice, <i>Biology</i>, 2020, 9 (10), pp.341. <a target="_blank" href="https://dx.doi.org/10.3390/biology9100341">⟨10.3390/biology9100341⟩</a>.
2020
Etzerodt A, Moulin M, Doktor TK, Delfini M, Mossadegh-Keller N, Bajenoff M, Sieweke MH, Moestrup SK,..., Tissue-resident macrophages in omentum promote metastatic spread of ovarian cancer. J Exp Med 2020 Apr; 217(4): .
2019
Cassol F, Portal L, Richelme S, Dupont M, Boursier Y, Arechederra M, Auphan-Anezin N, Chasson L, Lap..., Tracking Dynamics of Spontaneous Tumors in Mice Using Photon-Counting Computed Tomography. iScience 2019 Nov; 21(): 68-83.
2019
Reverendo M, Argüello RJ, Polte C, Valečka J, Camosseto V, Auphan-Anezin N, Ignatova Z, Gatti E, P..., Polymerase III transcription is necessary for T cell priming by dendritic cells. Proc Natl Acad Sci USA 2019 Nov; 116(45): 22721-22729.
2019
Gregory Verdeil, Toby Lawrence, Anne-Marie Schmitt-Verhulst, Nathalie Auphan-Anezin, Targeting STAT3 and STAT5 in Tumor-Associated Immune Cells to Improve Immunotherapy, <i>Contemporary Southeast Asia</i>, 2019, 11 (12), <a target="_blank" href="https://dx.doi.org/10.3390/cancers11121832">⟨10.3390/cancers11121832⟩</a>.
2019
Anders Etzerodt, Kyriaki Tsalkitzi, Maciej Maniecki, William Damsky, Marcello Delfini, Elodie Baudoi..., Specific targeting of CD163(+) TAMs mobilizes inflammatory monocytes and promotes T cell-mediated tumor regression, <i>Journal of Experimental Medicine</i>, 2019, 216 (10), pp.2394-2411. <a target="_blank" href="https://dx.doi.org/10.1084/jem.20182124">⟨10.1084/jem.20182124⟩</a>.
2017
Melissa Frick, Pierre Mouchacca, Grégory Verdeil, Yannick Hamon, Cyrille Billaudeau, Michel Buferne..., Distinct patterns of cytolytic T-cell activation by different tumor cells revealed by Ca2+ signaling and granule mobilization, <i>Immunology</i>, 2017, 150 (2), pp.199-212. <a target="_blank" href="https://dx.doi.org/10.1111/imm.12679">⟨10.1111/imm.12679⟩</a>.
2015
Giordano M, Henin C, Maurizio J, Imbratta C, Bourdely P, Buferne M, Baitsch L, Vanhille L, Sieweke M..., Molecular profiling of CD8 T cells in autochthonous melanoma identifies Maf as driver of exhaustion. EMBO J 2015 Aug; 34(15): 2042-58.
2015
Magali Grange, Marilyn Giordano, Amandine Mas, Romain Roncagalli, Guylène Firaguay, Jacques Nunes, ..., Control of CD8 T cell proliferation and terminal differentiation by active STAT5 and CDKN2A/CDKN2B. <i>Immunology</i>, 2015, 145 (4), pp.543-57. <a target="_blank" href="https://dx.doi.org/10.1111/imm.12471">⟨10.1111/imm.12471⟩</a>.
2015
Stéphanie O Morin, Valentin Giroux, Cédric Favre, Yassina Bechah, Nathalie Auphan-Anezin, Romain R..., In the absence of its cytosolic domain, the CD28 molecule still contributes to T cell activation, <i>Cellular and Molecular Life Sciences</i>, 2015, pp.DOI 10.1007/s00018-015-1873-7. <a target="_blank" href="https://dx.doi.org/10.1007/s00018-015-1873-7">⟨10.1007/s00018-015-1873-7⟩</a>.
2015
Buferne M, Chasson L, Grange M, Mas A, Arnoux F, Bertuzzi M, Naquet P, Leserman L, Schmitt-Verhulst ..., IFNγ producing CD8+ T cells modified to resist major immune checkpoints induce regression of MHC class I-deficient melanomas. Oncoimmunology 2015 Feb; 4(2): e974959.
2015
Auphan-Anezin N, Schmitt-Verhulst AM, Silence STAT3 in the procancer niche… and activate CD8+ T cells to kill premetastatic myeloid intruders. Eur J Immunol 2015 Jan;45(1):44-8.
2014
Giordano M, Roncagalli R, Bourdely P, Chasson L, Buferne M, Yamasaki S, Beyaert R, van Loo G, Auphan..., The tumor necrosis factor alpha-induced protein 3 (TNFAIP3, A20) imposes a brake on antitumor activity of CD8 T cells. Proc Natl Acad Sci USA 2014 Jul; 111(30): 11115-20.
2013
M. Grange, G. Verdeil, F. Arnoux, A. Griffon, Salvatore Spicuglia, J. Maurizio, M. Buferne, A.-M. Sc..., Active STAT5 Regulates T-bet and Eomesodermin Expression in CD8 T Cells and Imprints a T-bet-Dependent Tc1 Program with Repressed IL-6/TGF-1 Signaling, <i>Journal of Immunology</i>, 2013, 191 (7), pp.3712--3724. <a target="_blank" href="https://dx.doi.org/10.4049/jimmunol.1300319">⟨10.4049/jimmunol.1300319⟩</a>.
2013
Auphan-Anezin N, Verdeil G, Grange M, Soudja SM, Wehbe M, Buferne M, Mas A, Schmitt-Verhulst AM, Immunosuppression in inflammatory melanoma: can it be resisted by adoptively transferred T cells? Pigment Cell Melanoma Res 2013 Mar; 26(2): 167-75.
2012
Grange M, Buferne M, Verdeil G, Leserman L, Schmitt-Verhulst AM, Auphan-Anezin N, Activated STAT5 promotes long-lived cytotoxic CD8+ T cells that induce regression of autochthonous melanoma. Cancer Res 2012 Jan; 72(1): 76-87.
2007
Anil Shanker, Grégory Verdeil, Michel Buferne, Else-Marit Inderberg-Suso, Denis Puthier, Florence J..., CD8 T cell helps for innate antitumor immunity. Journal of Immunology , 2007, 179 (10), pp.6651-62.
2007
Catherine Mazza, Nathalie Auphan-Anezin, Claude Gregoire, Annick Guimezanes, Christine Kellenberger,..., How much can a T-cell antigen receptor adapt to structurally distinct antigenic peptides? <i>EMBO Journal</i>, 2007, 26 (7), pp.1972-83. <a target="_blank" href="https://dx.doi.org/10.1038/sj.emboj.7601605">⟨10.1038/sj.emboj.7601605⟩</a>.
2007
Nathalie Auphan-Anezin, Assymetric inheritance in the daughter cells determines effector or memory cell fate in T lymphocytes, Medicine/Sciences , 2007, 23 (8-9), pp.685-7.
2007
Bonnefoy-Bérard N, Auphan-Anezin N, Sautès-Fridman C, [The “rendez-vous” of the European immunologists in Paris]. Med Sci (Paris) 2007 Jan; 23(1): 105-6.
2006
Grégory Verdeil, Julie Chaix, Anne-Marie Schmitt-Verhulst, Nathalie Auphan-Anezin, Temporal cross-talk between TCR and STAT signals for CD8 T cell effector differentiation. <i>European Journal of Immunology</i>, 2006, 36 (12), pp.3090-100. <a target="_blank" href="https://dx.doi.org/10.1002/eji.200636347">⟨10.1002/eji.200636347⟩</a>.
2006
Auphan-Anezin N, Mazza C, Guimezanes A, Barrett-Wilt GA, Montero-Julian F, Roussel A, Hunt DF, Malis..., Distinct orientation of the alloreactive monoclonal CD8 T cell activation program by three different peptide/MHC complexes. Eur J Immunol 2006 Jul; 36(7): 1856-66.
2006
Grégory Verdeil, Denis Puthier, Catherine Nguyen, Anne-Marie Schmitt-Verhulst, Nathalie Auphan-Anez..., STAT5-mediated signals sustain a TCR-initiated gene expression program toward differentiation of CD8 T cell effectors. Journal of Immunology , 2006, 176 (8), pp.4834-42.
2004
Shanker A, Auphan-Anezin N, Chomez P, Giraudo L, Van den Eynde B, Schmitt-Verhulst AM, Thymocyte-intrinsic genetic factors influence CD8 T cell lineage commitment and affect selection of a tumor-reactive TCR. J Immunol 2004 Apr; 172(8): 5069-77.
2003
Auphan-Anezin N, Verdeil G, Schmitt-Verhulst AM, Distinct thresholds for CD8 T cell activation lead to functional heterogeneity: CD8 T cell priming can occur independently of cell division. J Immunol 2003 Mar; 170(5): 2442-8.
2002
G. Verdeil, Denis Puthier, Cong Tu Nguyen, AM Schmitt-Verhulst, N. Auphan-Anezin, Gene profiling approach to establish the molecular bases for partial versus full activation of naïve CD8 T lymphocytes, Annals of the New York Academy of Sciences , 2002, 975, pp.68--76.
2001
Auphan-Anezin N, Schmitt-Verhulst AM, Differential survival of transferred CD8 T cells and host reconstitution depending on TCR avidity for host-expressed alloantigen. J Immunol 2001 Jun; 166(12): 7200-7.
2000
Simon AK, Auphan N, Pophillat M, Boyer C, Ghosh S, Rincón M, Flavell RA, Schmitt-Verhulst AM, The lack of NF-kappa B transactivation and PKC epsilon expression in CD4(+)CD8(+) thymocytes correlates with negative selection. Cell Death Differ 2000 Dec; 7(12): 1253-62.
2000
Yang WC, Ghiotto M, Castellano R, Collette Y, Auphan N, Nunès JA, Olive D, Role of Tec kinase in nuclear factor of activated T cells signaling. Int Immunol 2000 Nov; 12(11): 1547-52.
1999
Auphan N, Ghosh S, Flavell RA, Schmitt-Verhulst AM, Differential requirements for NF-kappaB and AP-1 trans-activation in response to minimal TCR engagement by a partial agonist in naive CD8 T cells. J Immunol 1999 Nov; 163(10): 5219-27.
1998
Auphan N, Simon AK, Asnagli H, Phillips RJ, Rincon M, Ghosh S, Flavell RA, Schmitt-Verhulst AM, Consequences of intrathymic TCR engagement by partial agonist on selection events and peripheral T cell activation program., J Immunol 1998 May; 160(10): 4810-21.
1997
Auphan N, Didonato JA, Helmberg A, Rosette C, Karin M, Immunoregulatory genes and immunosuppression by glucocorticoids. Arch Toxicol Suppl 1997; 19(): 87-95.
1996
Simon AK, Auphan N, Schmitt-Verhulst AM, Developmental control of antigen-induced thymic transcription factors. Int Immunol 1996 Sep; 8(9): 1421-8.
1996
Bernard K, Auphan N, Granjeaud S, Victorero G, Schmitt-Verhulst AM, Jordan BR, Nguyen C, Multiplex messenger assay: simultaneous, quantitative measurement of expression of many genes in the context of T cell activation. Nucleic Acids Res 1996 Apr; 24(8): 1435-42.
1995
Anel A, Simon AK, Auphan N, Buferne M, Boyer C, Golstein P, Schmitt-Verhulst AM, Two signaling pathways can lead to Fas ligand expression in CD8+ cytotoxic T lymphocyte clones. Eur J Immunol 1995 Dec; 25(12): 3381-7.
1995
Auphan N, DiDonato JA, Rosette C, Helmberg A, Karin M, Immunosuppression by glucocorticoids: inhibition of NF-kappa B activity through induction of I kappa B synthesis. Science 1995 Oct; 270(5234): 286-90.
1995
Helmberg A, Auphan N, Caelles C, Karin M, Glucocorticoid-induced apoptosis of human leukemic cells is caused by the repressive function of the glucocorticoid receptor. EMBO J 1995 Feb; 14(3): 452-60.
1994
Auphan N, Curnow J, Guimezanes A, Langlet C, Malissen B, Mellor A, Schmitt-Verhulst AM, The degree of CD8 dependence of cytolytic T cell precursors is determined by the nature of the T cell receptor (TCR) and influences negative selection in TCR-transgenic mice. Eur J Immunol 1994 Jul; 24(7): 1572-7.
1994
Sponaas AM, Tomlinson PD, Antoniou J, Auphan N, Langlet C, Malissen B, Schmitt-Verhulst AM, Mellor A..., Induction of tolerance to self MHC class I molecules expressed under the control of milk protein or beta-globin gene promoters. Int Immunol 1994 Feb; 6(2): 277-87.
1994
Schönrich G, Alferink J, Klevenz A, Küblbeck G, Auphan N, Schmitt-Verhulst AM, Hämmerling GJ, Arn..., Tolerance induction as a multi-step process. Eur J Immunol 1994 Feb; 24(2): 285-93.
1993
Schönrich G, Strauss G, Müller KP, Dustin L, Loh DY, Auphan N, Schmitt-Verhulst AM, Arnold B, Häm..., Distinct requirements of positive and negative selection for selecting cell type and CD8 interaction. J Immunol 1993 Oct; 151(8): 4098-105.
1992
Auphan N, Jézo-Brémond A, Schönrich G, Hämmerling G, Arnold B, Malissen B, Schmitt-Verhulst AM, Threshold tolerance in H-2Kb-specific TCR transgenic mice expressing mutant H-2Kb: conversion of helper-independent to helper-dependent CTL. Int Immunol 1992 Dec; 4(12): 1419-28.
1992
Auphan N, Schönrich G, Malissen M, Barad M, Hämmerling G, Arnold B, Malissen B, Schmitt-Verhulst A..., Influence of antigen density on degree of clonal deletion in T cell receptor transgenic mice. Int Immunol 1992 Apr; 4(4): 541-7.
1991
Grégoire C, Rebaï N, Schweisguth F, Necker A, Mazza G, Auphan N, Millward A, Schmitt-Verhulst AM, ..., Engineered secreted T-cell receptor alpha beta heterodimers. Proc Natl Acad Sci USA 1991 Sep; 88(18): 8077-81.
1991
Auphan N, Boyer C, Andre P, Bongrand P, Schmitt-Verhulst AM, Biochemical and functional association between CD8 and H-2 at the surface of a T cell clone. Mol Immunol 1991 Aug; 28(8): 827-37.
1991
Hämmerling GJ, Schönrich G, Momburg F, Auphan N, Malissen M, Malissen B, Schmitt-Verhulst AM, Arno..., Non-deletional mechanisms of peripheral and central tolerance: studies with transgenic mice with tissue-specific expression of a foreign MHC class I antigen. Immunol Rev 1991 Aug; 122(): 47-67.
1991
Boyer C, Auphan N, Luton F, Malburet JM, Barad M, Bizozzero JP, Reggio H, Schmitt-Verhulst AM, T cell receptor/CD3 complex internalization following activation of a cytolytic T cell clone: evidence for a protein kinase C-independent staurosporine-sensitive step. Eur J Immunol 1991 Jul; 21(7): 1623-34.
1989
Boyer C, Auphan N, Gabert J, Blanc D, Malissen B, Schmitt-Verhulst AM, Comparison of phosphorylation and internalization of the antigen receptor/CD3 complex, CD8, and class I MHC-encoded proteins on T cells. Role of intracytoplasmic domains analyzed with hybrid CD8/class I molecules. J Immunol 1989 Sep; 143(6): 1905-14.
1989
Le Bouteiller P, Auphan N, Weill B, Lemonnier FA, Antibodies to HLA class I promoter-binding proteins in sera from patients with systemic lupus erythematosus. Eur J Immunol 1989 Apr; 19(4): 735-9.