Génomique – CIML

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About

Created in 2019, the CIML Genomics Platform provides the institute's researchers and scientific staff with cutting-edge technologies in transcriptomics and genomics, as well as dedicated expertise to support their projects. The platform is composed of scientific director Pierre Milpied (Inserm Research Director) and Laurine Gil (CNRS Study Engineer).

In 2024, the platform obtained the “Aix-Marseille Platform” quality label for a period of three years, issued by Aix Marseille University, Inserm and CNRS.

The missions of the CIML Genomics platform are:

Provide and maintain the necessary equipment: The genomics platform provides material and technological support by making various equipment available.
Train users : the platform offers training to users, allowing them to master the techniques necessary for their scientific projects.
Supporting experiments : the platform offers experimental support to assist users during complex manipulations, thus ensuring the quality and precision of experiments.
Develop new methods : continuous technological monitoring allows us to offer the most recent innovations and make available protocols based on bulk RNA-seq or single-cell RNA-seq technologies.
Assist with experimental design : the platform helps users to best define their experimental design, in close collaboration with the CIML CB2M (Computational Biology, Biostatistics and Modeling) platform.
Carry out projects : the platform offers to fully support certain genomics experiments to meet the specific needs of research projects (library preparation, sequencing).

Our Services

The genomics platform supports users throughout their projects, from experimental design to sequencing data acquisition, ensuring scientific rigor and technical quality. This support is offered through various services:

Experimental design : the platform provides advice and support in defining experimental design, in close coordination with all the other CIML platforms involved.
Training and experimental support : the platform assists users in carrying out complex experiments, ensuring the reliability and reproducibility of experiments.
Access to genomics equipment : the platform provides a wide range of instruments (thermal cyclers, qPCR devices, Agilent TapeStation, Chromium X).
Preparation of single-cell RNA-seq libraries : the platform supports the preparation of single-cell RNA-seq libraries at the request of project leaders.
High-throughput sequencing : the platform ensures the sequencing of libraries on the Illumina NextSeq 2000 sequencer.

Our Technologies

We have advanced expertise and the necessary equipment to perform multi-omic analyses on single cells after droplet capture (10x Genomics technology): single-cell RNA-seq, CITE-seq, single-cell BCR-seq, single-cell TCR-seq.

We are also experts in the single-cell multi-omic analysis technology FB5P-seq (FACS-based 5′-end sequencing), developed at CIML, and enabling the integrative analysis (phenotype, transcriptome, BCR or TCR repertoire) of rare cells sorted by FACS.

We provide the necessary equipment for the preparation and quality control of sequencing libraries for any type of protocol, and we perform the sequencing of compatible libraries on our Illumina NextSeq2000 high-throughput sequencer.

We have a fleet of 4 real-time quantitative PCR equipment, compatible with 96-well or 384-well formats.

Training

The genomics platform provides training activities at two levels:

Within the CIML, by providing staff training in techniques related to the preparation of single-cell RNAseq libraries.
Nationally, with Inserm training “Single-cell RNAseq analyses”, organized once or twice a year in collaboration with the CIML CB2M platform.

Members

About

Our Services

Our Technologies

Training

Projects

Equipments