Cytométrie

Members

Cyrille MIONNET

Research Officer

About

The cytometry platform: expertise and reliability at the service of research

The cytometry platform aims to support researchers in setting up robust and reproducible experiments. It constitutes a reference space where technical expertise and scientific support combine to guarantee the quality and reliability of data.

Founded in 1976 with the creation of the Immunology Center, the Cytometry Service operates with two engineers, Sylvain Bigot (department manager) and Laurence Borge. Scientific responsibility is assured by Cyrille MionnetThe service is assisted by a steering committee, made up of advanced users, which meets every month.

Our services are structured around four axes:

Development of data processing and preparation workflows, integrating quality control, compensation and standardization, for reliable analyses whatever the software environment.

Design and optimization of multiparametric panels, in order to produce data that can be used in conventional and multidimensional analyses.

Provision of validated protocols, covering the essential steps of sample preparation, antibody titration and the study of key cellular processes such as apoptosis and the cell cycle.

Standardization and monitoring of instruments, ensuring the stability and comparability of acquisitions over time.

The main missions of the service are as follows:

Cell sorting under biological conditions of any type of cells in any medium: 1.5 ml to 15 ml tube or multi-well plates (96 or 384 wells). Sorting is carried out under conditions optimizing cell viability.
Quality control and maintenance of instruments.
User training: Users are trained to become self-sufficient on all of the department's analyzers. The training consists of two modules: a theoretical module and a practical module on an analyzer. Beginner users are then assisted in starting their own experiments.
Advice and assistance for cytometry experiments, data analysis and cell sorting.
Evaluation of new cell analysis and sorting technologies to maintain cytometry at the highest level in the department.
Development and optimization of analyzers and sorters: modification of instruments for optimal detection of fluorescent probes and proteins.

The service has an online booking calendar and a network of servers for secure data storage.

Training

Beyond these services, the platform places training and support for users at the heart of its mission. It ensures that best practices are transmitted and applied, thus guaranteeing methodological rigor and reproducibility of results. Training adapted to the needs and cytometry level of each user is offered by the platform: Conventional multiparametric cytometry, spectral cytometry, cell sorting.

Open Science

Through this approach, the platform contributes directly to the scientific excellence of research projects and to strengthening the quality of data generated in cytometry.

Members

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Training

Open Science

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