Publication: Isolation and Characterization of Testis Macrophages Using Flow Cytometry.

Published in: Methods Mol Biol 2024 ; 2713(): 199-206

Authors: Sekias M, Baratin M, Bajenoff M

Summary

Testis-resident macrophages are first responders of the innate immune system against pathogens. They also exert day-to-day functions that are poorly understood. To study testis macrophages, several techniques are used, among which we can find flow cytometry.Flow cytometry is a powerful tool that enables analysis of macrophages at a cellular as well as population level. To analyze testis macrophages using flow cytometry, a specific tissue processing is necessary to extract them. In this protocol, we explain how to extract and analyze the distinct macrophage populations.

Link to Pubmed [PMID] – 37639125

Link to DOI – 10.1007/978-1-0716-3437-0_14