Publication: Nidogen-1 Contributes to the Interaction Network Involved in Pro-B Cell Retention in the Peri-sinusoidal Hematopoietic Stem Cell Niche

Published in: Cell Rep 2019 Mar; 26(12): 3257-3271.e8

Authors: Marielle Balzano, Maria de Grandis, Thien-Phong Vu Manh, Lionel Chasson, Florence Bardin, Anne Farina, Arnauld Sergé, Ghislain Bidaut, Pierre Charbord, Léonard Hérault, Anne-Laure Bailly, Amandine Cartier-Michaud, Annie Boned, Marc Dalod, Estelle Duprez, Paul Genever, Mark C Coles, Marc Bajenoff, Luc Xerri, Michel Aurrand-Lions, Claudine Schiff, Stéphane J.C. Mancini

Summary

In the bone marrow, CXCL12 and IL-7 are essential for B cell differentiation, whereas hematopoietic stem cell (HSC) maintenance requires SCF and CXCL12. Peri-sinusoidal stromal (PSS) cells are the main source of IL-7, but their characterization as a pro-B cell niche remains limited. Here, we characterize pro-B cell supporting stromal cells and decipher the interaction network allowing pro-B cell retention. Preferential contacts are found between pro-B cells and PSS cells, which homogeneously express HSC and B cell niche genes. Furthermore, pro-B cells are frequently located in the vicinity of HSCs in the same niche. Using an interactome bioinformatics pipeline, we identify Nidogen-1 as essential for pro-B cell retention in the peri-sinusoidal niche as confirmed in Nidogen-1-/- mice. Finally, human pro-B cells and hematopoietic progenitors are observed close to similar IL-7+ stromal cells. Thus, a multispecific niche exists in mouse and human supporting both early progenitors and committed hematopoietic lineages.

Link to Pubmed [PMID] – 30893599

Link to HAL – hal-03395907

Link to DOI – 10.1016/j.celrep.2019.02.065