Publication: SUnSET, a nonradioactive method to monitor protein synthesis.

Publié dans: Nat Methods 2009 Apr; 6(4): 275-7

Auteurs: Schmidt EK, Clavarino G, Ceppi M, Pierre P

Résumé

We developed a nonradioactive fluorescence-activated cell sorting-based assay, called surface sensing of translation (SUnSET), which allows the monitoring and quantification of global protein synthesis in individual mammalian cells and in heterogeneous cell populations. We demonstrate here, using mouse dendritic and T cells as a model, that SUnSET offers a technical alternative to classical radioactive labeling methods for the study of mRNA translation and cellular activation.

Lien vers Pubmed [PMID] – 19305406

Lien vers le DOI – 10.1038/nmeth.1314