Publication: Expanding the tools for identifying mononuclear phagocyte subsets in swine: Reagents to porcine CD11c and XCR1 Accroitre les outils pour identifier les sous-populations de phagocytes mononucléés chez le porc : Réactifs contre CD11c et XCR1

Publié dans: Dev Comp Immunol 2016 Dec; 65(): 31-40

Auteurs: Charlotte Deloizy, Edwige Bouguyon, Even Fossum, Peter Sebo, Radim Osicka, Angelique Bole, Michel Pierres, Stephane Biacchesi, Marc Dalod, Bjarne Bogen, Nicolas Bertho, Isabelle Schwartz-Cornil

Résumé

Pig is a domestic species of major importance in the agro-economy and in biomedical research. Mononuclear phagocytes (MNP) are organized in subsets with specialized roles in the orchestration of the immune response and new tools are awaited to improve MNP subset identification in the pig. We cloned pig CD11c cDNA and generated a monoclonal antibody to pig CD11c which showed a pattern of expression by blood and skin MNP subsets similar to humans. We also developed a porcine XCL1-mCherry dimer which specifically reacted with the XCR1-expressing dendritic cell subset of the type 1 lineage in blood and skin. These original reagents will allow the efficient identification of pig MNP subsets to study their role in physiological and pathological processes and also to target these cells in novel intervention and vaccine strategies for veterinary applications and preclinical evaluations. (C) 2016 Elsevier Ltd. All rights reserved.

Lien vers Pubmed [PMID] – 27345169

Lien vers HAL – hal-01438164

Lien vers le DOI – 10.1016/j.dci.2016.06.015