Publication: Deletion of the LIME adaptor protein minimally affects T and B cell development and function.

Publié dans: European Journal of Immunology, 2007, 37 (11), pp.3259-69. ⟨10.1002/eji.200737563⟩

Auteurs: Claude Grégoire, Sarka Simova, Ying Wang, Amandine Sansoni, Sylvie Richelme, Anja Schmidt-Giese, Luca Simeoni, Pavla Angelisova, Dirk Reinhold, Burkhart Schraven, Vaclav Horejsi, Bernard Malissen, Marie Malissen

Résumé

LIME (Lck-interacting membrane protein) is a transmembrane adaptor that associates with the Lck and Fyn protein tyrosine kinases and with the C-terminal Src kinase (Csk). To delineate the role of LIME in vivo, LIME-deficient mice were generated. Although Lime transcripts were expressed in immature and mature B and T cells, the absence of LIME impeded neither the development nor the function of B and T cells. TCR transgenic mice deprived of LIME showed, however, a 1.8-fold enhancement in positive selection. Since B cells and activated T cells express LIME and the related adaptor NTAL, mice lacking both adaptors were generated. Double-deficient mice showed no defect in the development and function of B and T cells, and the lack of LIME had no effect on the autoimmune syndrome that develops in aged NTAL-deficient mice. In contrast to a previous report, we further showed that this autoimmune syndrome develops in the absence of T cells. Therefore, our in vivo results refute all the previous roles postulated for LIME on the basis of studies of transformed B and T cells and demonstrate that LIME has no seminal role in the signaling cassette operated by antigen receptors and coreceptors.

Lien vers HAL – hal-00297265

Lien vers le DOI – 10.1002/eji.200737563