
Publication: Cell metabolism: Functional and phenotypic single cell approaches
Publié dans: Methods in Cell Biology, 2024, Methods in Cell Biology, 186, pp.151-187. ⟨10.1016/bs.mcb.2024.02.024⟩
Auteurs: Sara de Biasi, Julien Paul Gigan, Rebecca Borella, Elena Santacroce, Domenico Lo Tartaro, Anita Neroni, Nikolaos Paschalidis, Katarzyna Piwocka, Rafael José Argüello, Lara Gibellini, Andrea Cossarizza
Résumé
Several metabolic pathways are essential for the physiological regulation of immune cells, but their dysregulation can cause immune dysfunction. Hypermetabolic and hypo-metabolic states represent deviations in the magnitude and flexibility of effector cells in different contexts, for example in autoimmunity, infections or cancer. To study immu-nometabolism, most methods focus on bulk populations and rely on in vitro activation assays. Nowadays, thanks to the development of single-cell technologies, including multiparameter flow cytometry, mass cytometry, RNA cytometry, among others, the metabolic state of individual immune cells can be measured in a variety of samples obtained in basic, translational and clinical studies. Here, we provide an overview of dif-ferent single-cell approaches that are employed to investigate both mitochondrial func-tions and cell dependence from mitochondria metabolism. Moreover, besides the description of the appropriate experimental settings, we discuss the strengths and weaknesses of different approaches with the aim to suggest how to study cell metab-olism in the settings of interest.
AKT serine/threonine kinase adenosine triphosphate mitochondrial ATP synthase boronophenylalanine carnitine palmitoyltransferase 1a citrate synthase cytochrome C fatty acids oxidation glucose transporters hypoxia inducible factor 1 subunit alpha 5,5 0 ,6,6 0 -tetrachloro-1,1 0 ,3,3 0 -tetraethyl-benzimidazol-carbocyanine L-type amino acid transporter 1 median fluorescence intensity mitochondria peroxy yellow-1 MitoSOX red mitochondrial superoxide indicator mechanistic target of rapamycin kinase nuclear factor kappa B mitochondrial oxidative phosphorylation phospho acetyl-CoA carboxylase phosphatidylinositol-4,5-bisphosphate 3-kinase puromycin single-cell metabolic regulome profiling T cell receptor tetramethylrhodamine ethyl ester tetramethylrhodamine methyl ester voltage dependent anion channel
Lien vers HAL – amu-04943185
Lien vers le DOI – 10.1016/bs.mcb.2024.02.024