Publication: An inducible model for specific neutrophil depletion by diphtheria toxin in mice.

Publié dans: Sci China Life Sci 2021 Aug; 64(8): 1227-1235

Auteurs: Chao T, Lu L, Zhang L, Huang R, Liu Z, Zhou B, Kong E, Zhang Z, Lawrence T, Liang Y

Résumé

Neutrophils are crucial for immunity and play important roles in inflammatory diseases; however, mouse models selectively deficient in neutrophils are limited, and neutrophil-specific diphtheria toxin (DT)-based depletion system has not yet been established. In this study, we generated a novel knock-in mouse model expressing diphtheria toxin receptor (DTR) under control of the endogenous Ly6G promoter. We showed that DTR expression was restricted to Ly6G+ neutrophils and complete depletion of neutrophils could be achieved by DT treatment at 24-48 h intervals. We characterized the effects of specific neutrophil depletion in mice at steady-state, with acute inflammation and during tumor growth. Our study presents a valuable new tool to study the roles of neutrophils in the immune system and during tumor progression.

Lien vers Pubmed [PMID] – 33420927

Lien vers le DOI – 10.1007/s11427-020-1839-3