Publication: Barcoding T cell calcium response diversity with methods for automated and accurate analysis of cell signals (MAAACS).

Publié dans: PLoS Comput Biol 2013 ; 9(9): e1003245

Auteurs: Salles A, Billaudeau C, Sergé A, Bernard AM, Phélipot MC, Bertaux N, Fallet M, Grenot P, Marguet D, He HT, Hamon Y

Résumé

We introduce a series of experimental procedures enabling sensitive calcium monitoring in T cell populations by confocal video-microscopy. Tracking and post-acquisition analysis was performed using Methods for Automated and Accurate Analysis of Cell Signals (MAAACS), a fully customized program that associates a high throughput tracking algorithm, an intuitive reconnection routine and a statistical platform to provide, at a glance, the calcium barcode of a population of individual T-cells. Combined with a sensitive calcium probe, this method allowed us to unravel the heterogeneity in shape and intensity of the calcium response in T cell populations and especially in naive T cells, which display intracellular calcium oscillations upon stimulation by antigen presenting cells.

Lien vers Pubmed [PMID] – 24086124

Lien vers HAL – hal-01876218

Lien vers le DOI – 10.1371/journal.pcbi.1003245