Publication: Cutting edge: Priming of NK cells by IL-18.

Publié dans:

Auteurs: Julie Chaix, Marlowe S Tessmer, Kasper Hoebe, Nicolas Fuséri, Bernhard Ryffel, Marc Dalod, Lena Alexopoulou, Bruce Beutler, Laurent Brossay, Eric Vivier, Thierry Walzer

Résumé

Recent evidence suggests that NK cells require priming to display full effector activity. In this study, we demonstrate that IL-18 contributed to this phenomenon. IL-18 signaling-deficient NK cells were found to be unable to secrete IFN-gamma in response to ex vivo stimulation with IL-12. This was not due to a costimulatory role of IL-18, because blocking IL-18 signaling during the ex vivo stimulation with IL-12 did not alter IFN-gamma production by wild-type NK cells. Rather, we demonstrate that IL-18 primes NK cells in vivo to produce IFN-gamma upon subsequent stimulation with IL-12. Importantly, IL-12-induced IFN-gamma transcription by NK cells was comparable in IL-18 signaling-deficient and -sufficient NK cells. This suggests that priming by IL-18 leads to an improved translation of IFN-gamma mRNA. These results reveal a novel type of cooperation between IL-12 and IL-18 that requires the sequential action of these cytokines.

Lien vers Pubmed [PMID] – 18641298

Lien vers HAL – hal-00363717